Nterminal
Sequence
Analysis
Ala22
Structure / Form Disulfidelinked
homodimer
Predicted Molecular
Mass
66.3 kDa
SPECIFICATIONS
SDSPAGE
7075
kDa, reducing conditions
Activity Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. （2003） Reproduction 125:271.
Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL, 100 μL/well, will mediate >25% BALB/3T3 cell adhesion （3 x 104
cell/well added）.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >90%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Cyr61, also known as CCN1, is a 40 45
kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration （1）. Cyr61 consists of an
IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain （2）. Mature human Cyr61 shares 93% amino acid sequence identity with
mouse and rat Cyr61. It is widely expressed during development and in adult tissues （2, 3）. Cyr61 associates with the extracellular matrix （ECM） and with many cell
surface molecules including Integrins αVβ3, αVβ5, αMβ2, and α6β1, Syndecan4,
and heparan sulfate proteoglycans （1, 3）. Cyr61 mediates the adhesion and migration
of multiple cell types and also promotes vascular endothelial cell tubule formation （4 6）.
Plasmin cleavage of ECMbound
Cyr61 releases a 28 kDa Nterminal
fragment which retains the ability to promote endothelial cell migration （7）. Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and
promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma （8 - 12）. In contrast,
when down-regulated, it suppresses tumor growth in endometrial, hepatic, and nonsmall
cell lung cancers （8, 13, 14）. Cyr61 is also up-regulated in injured skin and
bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair （15, 16）.
References:
1. Chen, Y. and X.Y. Du （2007） J. Cell. Biochem. 100:1337.
2. Jay, P. et al. （1997） Oncogene 14:1753.
3. Feng, P. et al. （2008） Int. J. Biochem. Cell Biol. 40:98.
4. Chen, N. et al. （2000） J. Biol. Chem. 275:24953.
5. Leu, S.J.
et al. （2002） J. Biol. Chem. 277:46248.
6. Schutze, N. et al. （2007） BMC Cell Biol. 8:45.
7. Pendurthi, U.R. et al. （2005） Cancer Res. 65:9705.
8. O'Kelly, J. et al. （2008） Int. J. Oncol. 33:59.
9. Chintalapudi, M.R. et al. （2008） Carcinogenesis 29:696.
10. Xie, D. et al. （2004） Cancer Res. 64:1987.
11. Lin, M.T.
et al. （2007） J. Biol. Chem. 282:34594.
12. Monnier, Y. et al. （2008） Cancer Res. 68:7323.
13. Tong, X. et al. （2001） J. Biol. Chem. 276:47709.
14. Chien, W. et al. （2004） J. Biol. Chem. 279:53087.
15. Hadjiargyrou, M. et al. （2000） J. Bone Miner. Res.15:1014.
16. Chen, C.C.
et al. （2001） J. Biol. Chem. 276:47329.
Recombinant Human Cyr61/CCN1
Fc Chimera
Catalog Number: 4055-CR
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