對M-MLV RTase進(jìn)行了改良 提高了延伸性?反應(yīng)效率?高溫反應(yīng)性
ReverTra Ace是在M-MLV RTase基因的RNaseH活性區(qū)域和DNA合成區(qū)域中導(dǎo)入了本公司*的點(diǎn)突變、
改變了酶機(jī)能的高性能逆轉(zhuǎn)錄酶。實(shí)現(xiàn)了*的高溫反應(yīng)性、延伸性和反應(yīng)效率。
ＲｅｖｅｒＴｒａ?。粒悖?br />（MMLV Reverse Transcriptase RNaseH-）
Code No. TRTLot
No. ******
Storage Store at -20℃
Size 10,000units（ 101） 50,000units（ 102）
Source ： Escherichia coli DH5?? （pDV68）
Concentration : １００ units/μl
Unit Definition : One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into
acid insoluble fraction in 10 minutes at 42℃ when poly（A）+ RNA and oligo （dT）20
are used as template-primer.
Assay Condition : 50 mM Tris-HCl（pH8.3）
75 mM KCl
3 mM MgCl2
10 mM DTT
240 μg/ml poly（A）+ RNA
0.1 μM oligo （dT）20
1 mM dTTP
0.3 μM [methyl-3H]dTTP
Storage Buffer : 20 mM Tris-HCl（pH7.5）
100 mM NaCl
0.1 mM EDTA
10 mM 2-mercaptoethanol
0.01 % NP-40
50 % Glycerol
5 X Buffer : Not containing dNTPs. cDNA synthesis reaction requires1mM of dNTPs.
Quality Control
1. RNase Activity : When 50 units of this enzyme were incubated with 0.5 μg of MS2 RNA for 1 hour
at 42℃, RNase activity was not observed after agarose gel electrophoresis.
Remarks Research Use Only. Not for Human Use
上海力敏實(shí)業(yè)有限公司代理TOYOBO產(chǎn)品，咨詢
上海力敏實(shí)業(yè)有限公司代理TOYOBO產(chǎn)品，咨詢
| RNaseH活性在cDNA合成中，會(huì)分解作為模板/引物的DNA-RNA雜交體中的RNA，導(dǎo)致反 |
